Journal: Cellular and Molecular Bioengineering

Article Title: Re-engineering Antimicrobial Peptides into Oncolytics Targeting Drug-Resistant Ovarian Cancers

doi: 10.1007/s12195-020-00626-z

Figure Lengend Snippet: Chemotherapeutic synergy. (a) Isobolograms of MAD1 and Doxorubicin (Dox), Paclitaxel (Ptx) or Cisplatin (Cis) combinatorial synergy in OVCAR-3 (left) and NCI/ADR-RES (right) cells. Fractional inhibitory concentration (FIC) < 1 and < 0.5 represent additive and synergistic effects, respectively. (b) Comparison of Dox and Cis IC50 towards NCI/ADR-RES as either a monotherapy (ADR-RES, black) or in combination with 20 μM MAD1 (ADR-RES + MAD1, grey). Activity of each drug as a monotherapy in pre-resistant OVCAR-3 (OVCAR-3, white) cells shown for comparison. (c–f) Indicated ovarian cancer cell line or patient-derived ovarian carcinoma cells were cultured in ultra-low attachment conditions and treated for 48 h with 2 μM cisplatin or 4 μM MAD1 alone and in combination. Data represent volume in arbitrary units (a.u.) and median.

Article Snippet: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Fmoc-protected amino acids, N,N 0 -diisopropylcarbodiimide (DIC), O-(7-Azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU), N,N -dimethylformamide (DMF), and Paclitaxel were purchased from Chem-Impex International, Inc. RPMI-1640, M199, trypsin and EDTA solution, Antibiotic-Antimycotic Solution, ultra-low attachment (ULA) plates, and 1× phosphate buffered saline (PBS) were purchased from Corning.

Techniques: Concentration Assay, Comparison, Activity Assay, Derivative Assay, Cell Culture

Journal: Antimicrobial Agents and Chemotherapy

Article Title: A New Natural Product Analog of Blasticidin S Reveals Cellular Uptake Facilitated by the NorA Multidrug Transporter

doi: 10.1128/AAC.02635-16

Figure Lengend Snippet: The effect of norA on resistance and cellular uptake for blasticidin S (BlaS), P10, and norfloxacin (NOR). (A) Disk diffusion assay showing zones of inhibition surrounding paper disks loaded with antibiotic overlaid on bacterial cultures of S. aureus TCH1516 WT (panel i), TCH1516 P10r-16 (ΔnorA) (panel ii), TCH1516 P10r-16::norA (norA complementation) exposed to blasticidin S (panel iii), TCH1516 WT (panel iv), TCH1516 P10r-16 (panel v), TCH1516 P10r-16::norA exposed to P10 (panel vi), TCH1516 WT (panel vii), TCH1516 P10r-16 (panel viii), and TCH1516 P10r-16::norA exposed to norfloxacin (panel ix). (B) E. coli NEB5α (left panel) and NEB5α::norA (right panel) exposed to blasticidin S (panel i), P10 (panel ii), and norfloxacin (panel iii). (C) Quantitation of the concentration of blasticidin, P10, and norfloxacin in cell lysates of E. coli NEB5α transformed with a control plasmid or the norA gene, exposed to each antibiotic; error bars represent standard deviations of results from three replicates.

Article Snippet: Blasticidin S and norfloxacin were purchased from Chem-Impex (Wood Dale, IL, USA), ampicillin, nisin, and reserpine from Sigma-Aldrich (St. Louis, MO, USA), erythromycin from Alfa-Aesar (Tewksbury, MA, USA), and vancomycin from Amresco (Solon, OH, USA).

Techniques: Diffusion-based Assay, Inhibition, Quantitation Assay, Concentration Assay, Transformation Assay, Plasmid Preparation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: A New Natural Product Analog of Blasticidin S Reveals Cellular Uptake Facilitated by the NorA Multidrug Transporter

doi: 10.1128/AAC.02635-16

Figure Lengend Snippet: The MIC of blasticidin S, P10, and norfloxacin against S. aureus strains with modified norA expression levels

Article Snippet: Blasticidin S and norfloxacin were purchased from Chem-Impex (Wood Dale, IL, USA), ampicillin, nisin, and reserpine from Sigma-Aldrich (St. Louis, MO, USA), erythromycin from Alfa-Aesar (Tewksbury, MA, USA), and vancomycin from Amresco (Solon, OH, USA).

Techniques: Modification, Expressing

Journal: Frontiers in Veterinary Science

Article Title: Antibiotic Usage Pattern in Broiler Chicken Flocks in Germany

doi: 10.3389/fvets.2021.673809

Figure Lengend Snippet: Relative TF per antimicrobial class and fattening week.

Article Snippet: Since 2012, the private company QS (QS Qualität und Sicherheit GmbH) has run an antibiotic monitoring system for its members.

Techniques: